General description

Full-length, recombinant, human UbcH5c expressed in E. coli. UbcH5c, an E2 ubiquitin ligase, is highly homologous to the yeast UbC4/5 family of E2 enzymes. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of transcription factors such as p53, NF-κB3, and c-Fos. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Brzovic, P.S., et al. 2006. Cell Cycle5, 2867.Gehrke, S.G., et al. 2003 Blood.101, 3288.Nuber, U., And Scheffner, M., 1999. J. Biol. Chem.274, 7576.Schwarz, S.E., et al. 1998. J. Biol. Chem.273, 12148.Jensen, J., et al. 1995. J. Biol. Chem.270, 30408.Scheffner, M., et al. 1994. Proc. Natl. Acad. Sci.91, 8797.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM HEPES, 50 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)